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Related Experiment Video

Updated: Jan 7, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Multimodal Single Extracellular Vesicle Profiling Technologies to Unveil Clinical Heterogeneity.

Yujin Kwon¹, Hyemin Kim¹, Hyun Kwon¹

¹Department of Integrative Biotechnology, Kookmin University, Seoul 02707, Republic of Korea.

ACS Applied Bio Materials

|December 29, 2025

Summary

Single extracellular vesicle (EV) profiling overcomes heterogeneity challenges for disease diagnosis. New multimodal platforms enable digital quantification of surface proteins and transcriptomes for advanced EV analysis.

Keywords:

heterogeneity high-throughput multimodal multiplexed single extracellular vesicles ultrasensitive

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Last Updated: Jan 7, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Published on: March 17, 2023

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

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Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

Area of Science:

Biochemistry
Molecular Biology
Nanotechnology

Background:

Extracellular vesicles (EVs) are crucial in disease via molecular cargo (proteins, transcriptomes, metabolites).
EV heterogeneity from diverse cell origins hinders clinical applications like diagnosis and therapeutics.
Current single EV profiling is mainly limited to qualitative/semiquantitative surface protein analysis.

Purpose of the Study:

To review qualitative and semiquantitative surface protein profiling methods for EVs.
To highlight advances in multimodal single EV profiling platforms.
To discuss the future outlook and challenges of multiomics single EV profiling.

Main Methods:

Review of existing qualitative and semiquantitative surface protein profiling techniques for EVs.
Analysis of recent multimodal single EV profiling platforms.
Discussion of technological advancements enabling digital quantification and multiomics analysis.

Main Results:

Multimodal platforms now allow digital quantification of surface proteins on single EVs.
Emerging technologies can detect the internal transcriptome of single EVs.
Simultaneous profiling of surface proteins and internal transcriptomes is now achievable.

Conclusions:

Single EV profiling technologies are advancing to address heterogeneity.
Multimodal platforms offer enhanced capabilities for comprehensive single EV analysis.
Technological hurdles remain, particularly in detecting small molecules within EVs.