Related Experiment Video
Updated: Jan 7, 2026

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Published on: October 20, 2021
Emergence of novel QX-type IBV in China: molecular insights into unique S protein cleavage sites, antigenic drift,
Jiaqi Zhang1, Jianjie Ren1, Wenqi Wu1
1Guangdong Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Key Laboratory of Zoonoses Prevention and Control (Guangdong Province), Key Laboratory of Animal Vaccine Development (Ministry of Agriculture), College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
Abstract:
Avian infectious bronchitis virus (IBV) is a major pathogen impacting the global poultry industry. The QX genotype (GI-19 lineage) of IBV has rapidly spread worldwide and is now the dominant genotype in Asia and Europe. In this study, three QX-type field strains (JS/773, JS/774, and SD/783) were isolated from diseased chicken flocks in eastern China, which had been vaccinated with IBV live attenuated vaccines (H120 or QXL87) between December 2024 and January 2025. Notably, the JS/773 strain showed an H536P mutation at the S protein cleavage site, marking the first identification of a PRRRR cleavage motif in this lineage and highlighting the diversity of cleavage sites among QX-type strains. Recombination analysis showed that these isolates are recombinant variants from vaccine strains 4/91, H120, and QXL87, as well as circulating field strains, with recombination occurring in the ORF1a/ORF1b, ORF5a/ORF5b and M regions. Pathogenicity testing in SPF chickens demonstrated that the isolates induced marked lesions in the respiratory and urinary systems; however, JS/773 caused the most severe tissue damage and resulted in the highest mortality rate among the groups. Cross-neutralization assays revealed substantial antigenic differences between the isolates and the H120 strain, and with reduced antigenic relatedness to the QXL87 strain. Seven amino acid mutations occurred in the isolates S1 subunit neutralizing epitope region, altering protein conformation and potentially contributing to antigenic variation and immune evasion. In conclusion, the genetic traits and pathogenicity of these isolates highlight the evolving QX-type strains in China, that offers new insights into the molecular evolution of QX-type IBV antigenicity.
Related Concept Videos
Viral Recombination
Single Nucleotide Polymorphisms-SNPs
Viral Mutations

