Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replicative Cell Senescence02:15

Replicative Cell Senescence

4.3K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.3K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.5K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Absence of GDF15 Aggravates Pressure Overload-Induced Cardiac Remodelling in Mice Hallmarked by Perivascular Fibrosis and Signs of Endothelial-to-Mesenchymal Transition.

International journal of molecular sciences·2026
Same author

Extracellular Vesicles Released by Picornavirus-Infected Cells Modify Antiviral Immune Cell Responses.

Journal of extracellular vesicles·2026
Same author

Efficiency and immunogenicity of lipid nanoparticle-mediated cardiac mRNA delivery are lipid composition-dependent.

Molecular therapy. Nucleic acids·2026
Same author

Author Correction: Engineering anti-BCMA CAR T cells for enhancing myeloma killing efficacy via apoptosis regulation.

Nature communications·2026
Same author

EV-Mediated Intracardiac Crosstalk Mitigates Doxorubicin-Induced Cardiotoxicity.

Circulation research·2026
Same author

Photoporation enables non-viral delivery of prime editing RNP complexes into human iPSC-derived cardiomyocytes for cardiac genome correction.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026

Related Experiment Video

Updated: Jan 7, 2026

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

7.7K

Enhancing cardiomyocyte reprogramming efficiency by targeting cellular senescence is mediated via Rb1 gene.

Juntao Fang1,2,3, Qiangbing Yang2,4, Renée G C Maas2,3

  • 1Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510080, China.

Stem Cell Research & Therapy
|December 30, 2025
PubMed
Summary

Cellular senescence impedes direct cardiac reprogramming of fibroblasts into cardiomyocytes. Inhibiting senescence-related genes, like RB1, significantly enhances this process, offering new avenues for cardiac repair strategies.

Keywords:
Direct cardiac reprogrammingRB1Senescence

More Related Videos

Assessing Cardiac Reprogramming using High Content Imaging Analysis
06:02

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

1.4K
Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
10:05

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

9.1K

Related Experiment Videos

Last Updated: Jan 7, 2026

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

7.7K
Assessing Cardiac Reprogramming using High Content Imaging Analysis
06:02

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

1.4K
Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
10:05

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

9.1K

Area of Science:

  • Cardiovascular Research
  • Cell Biology
  • Regenerative Medicine

Background:

  • Direct reprogramming of fibroblasts into cardiomyocytes using Gata4, Mef2c, and Tbx5 (GMT) shows promise for cardiac repair.
  • Low reprogramming efficiency is a major challenge, potentially influenced by cellular senescence.

Purpose of the Study:

  • To investigate the role of cellular senescence in direct cardiac reprogramming.
  • To identify senescence-related genes that act as barriers to reprogramming.
  • To assess the impact of targeting these genes on reprogramming efficiency.

Main Methods:

  • Established an inducible GMT expression system in mouse embryonic fibroblasts (MEFs) and human fetal cardiac fibroblasts (hFCFs).
  • Utilized RNA sequencing to identify senescence-related genes during reprogramming.
  • Knocked down identified senescence-related genes using shRNA and assessed reprogramming efficiency.

Main Results:

  • Direct cardiac reprogramming induced cellular senescence and apoptosis.
  • RNA sequencing identified RB1, RBBP4, RBBP7, CBX8, and CDKN1B as upregulated senescence-related genes.
  • Knockdown of these genes, especially RB1, significantly enhanced reprogramming efficiency and cardiac marker expression.

Conclusions:

  • Cellular senescence acts as a barrier to direct cardiac reprogramming.
  • Targeting senescence-related genes, particularly RB1, can improve reprogramming efficiency.
  • Findings provide novel insights into the regulation of direct cardiac reprogramming.