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The effect of L-ascorbate on catecholamine biosynthesis.

K J Stone, B H Townsley

    The Biochemical Journal
    |March 1, 1973
    PubMed
    Summary
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    Vitamin C (l-ascorbate) aids in phenylalanine hydroxylation by regenerating tetrahydrobiopterin. This vitamin may regulate key enzyme activities in vivo, impacting neurotransmitter synthesis.

    Area of Science:

    • Biochemistry
    • Enzymology
    • Nutritional Science

    Background:

    • Tetrahydrobiopterin is a crucial cofactor for aromatic amino acid hydroxylases.
    • Phenylalanine, tyrosine, and tryptophan hydroxylases are key enzymes in neurotransmitter and protein metabolism.
    • The role of l-ascorbate in the regulation of these hydroxylases has been investigated.

    Purpose of the Study:

    • To investigate the in vitro effect of l-ascorbate on phenylalanine hydroxylation.
    • To elucidate the mechanism by which l-ascorbate influences tetrahydrobiopterin recycling.
    • To explore the potential in vivo implications of ascorbate's action on hydroxylase activity.

    Main Methods:

    • In vitro enzymatic assays measuring phenylalanine hydroxylation.
    • Monitoring of tetrahydrobiopterin redox state and oxygen consumption during the reaction.

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  • Analysis of the interaction between l-ascorbate and the hydroxylation system.

    • Main Results:

    • l-Ascorbate was found to stimulate enzymic phenylalanine hydroxylation in vitro.
    • The stimulation is attributed to the recycling of tetrahydrobiopterin by l-ascorbate.
    • Oxygen consumption, essential for the reaction, was reduced due to cofactor regeneration.

    Conclusions:

    • l-Ascorbate plays a role in the cofactor recycling of tetrahydrobiopterin during phenylalanine hydroxylation.
    • This mechanism suggests a potential in vivo regulatory function for vitamin C in tyrosine and tryptophan hydroxylase activities.
    • Ascorbate's influence on hydroxylase activity may have implications for neurotransmitter biosynthesis and overall metabolic regulation.