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Related Experiment Video

Updated: May 13, 2026

Rapid High Throughput Amylose Determination in Freeze Dried Potato Tuber Samples
06:57

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Determining Irradiation Dose in Potato Tubers During Storage Using Reaction-Based Pattern Recognition Method.

Yana V Zubritskaya1,2, Anna V Shik3, Irina A Stepanova3

  • 1Skobeltsyn Institute of Nuclear Physics, Lomonosov Moscow State University, GSP-1, 1-2 Leninskiye Gory, 119991 Moscow, Russia.

Foods (Basel, Switzerland)
|December 30, 2025
PubMed
Summary

A new chemical fingerprinting method accurately estimates food irradiation doses in potatoes, even after days of storage. This simple, rapid technique offers a cost-effective alternative to traditional, complex instruments for dose monitoring.

Keywords:
chemometricsdose determinationfluorimetryfood irradiationindicator reactionphotometrypotatoes

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Area of Science:

  • Food science
  • Analytical chemistry
  • Radiation chemistry

Background:

  • Food irradiation extends shelf life and controls pests but requires accurate dose monitoring.
  • Current dose monitoring methods are expensive, laborious, and may miss low doses.
  • Previous chemical fingerprinting methods were limited to freshly irradiated samples.

Purpose of the Study:

  • To investigate the feasibility of a chemical fingerprinting method for determining irradiation dose magnitude in raw potatoes after storage.
  • To assess the method's accuracy and reliability over time and at different dose levels.

Main Methods:

  • Raw potato tubers were subjected to different irradiation doses (0, 100, 1000 Gy).
  • Water extracts were analyzed using oxidation-reduction and aggregation reactions in 96-well plates.
  • Reaction rates were tracked via absorbance and fluorescence, with chemometric analysis (SoftMax regression, LDA, PLS-DA).

Main Results:

  • The method successfully distinguished between dose orders of magnitude (0, 100, 1000 Gy) after 0, 2, and 6 days of storage at 4 °C.
  • Accuracy of dose recognition reached at least 97% on day 6 using SoftMax regression or LDA.
  • Partial least square-discriminant analysis confidently distinguished irradiated from non-irradiated samples.

Conclusions:

  • The reaction-based chemical fingerprinting method is feasible for assessing irradiation dose magnitude in stored potato tubers.
  • This simple, rapid, and equipment-light approach provides a viable alternative for post-treatment dose monitoring in food irradiation.