Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multiple Allele Traits01:49

Multiple Allele Traits

37.8K
The Concept of Multiple Allelism
37.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hashimoto's Thyroiditis Beyond Thyroid Hormones: A Systematic Review of Autoimmunity, Inflammation, and Multidimensional Burden.

Clinical endocrinology·2026
Same author

17q12 Recurrent Deletion Syndrome in Childhood.

Genes·2025
Same author

β-Lactam/β-Lactamase Inhibitor Combinations in Sepsis-Associated Acute Kidney Injury and Renal Replacement Therapy.

Antibiotics (Basel, Switzerland)·2025
Same author

Imprinting Disorders and Epigenetic Alterations in Children Conceived by Assisted Reproductive Technologies: Mechanisms, Clinical Outcomes, and Prenatal Diagnosis.

Genes·2025
Same author

Eosinophil ETosis and Cancer: Ultrastructural Evidence and Oncological Implications.

Cancers·2025
Same author

Management of Nephrotic Syndrome in Pediatric Patients Treated by Different Steroid Regimens.

Medicina (Kaunas, Lithuania)·2025

Related Experiment Video

Updated: Jan 7, 2026

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
10:37

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

3.3K

Host Immunogenetics and Chronic HCV Infection Shape Atopic Risk in Pediatric Beta-Thalassemia: A Genotype-Phenotype

Caterina Cuppari1, Alessio Mancuso2, Laura Colavita1

  • 1Pediatric Emergency Unit, Department of Maternal and Child Health, University Hospital of Messina, "G. Martino" Policlinic, 98124 Messina, Italy.

Genes
|December 30, 2025
PubMed
Summary

Pediatric beta-thalassemia patients with Hepatitis C virus (HCV) show reduced allergic sensitization and lower IgE levels, potentially due to immune tolerance influenced by IL10 and TLR7 gene variations. Further research is needed.

Keywords:
HCVIL10IgETLR7atopychronic infectiongene–environment interactionimmune regulationpediatric immunitythalassemia

More Related Videos

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
11:36

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms

Published on: May 29, 2020

3.3K
Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

2.8K

Related Experiment Videos

Last Updated: Jan 7, 2026

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
10:37

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

3.3K
Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
11:36

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms

Published on: May 29, 2020

3.3K
Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

2.8K

Area of Science:

  • Immunology
  • Pediatric Hematology
  • Infectious Diseases

Background:

  • Pediatric beta-thalassemia (BT) patients face immune challenges from transfusions and viral infections.
  • Hepatitis C virus (HCV) is common in polytransfused individuals and may alter immune responses.
  • The combined impact of HCV and host genetics on allergic sensitization in BT is not well understood.

Purpose of the Study:

  • To compare total serum IgE levels and allergic manifestations in HCV-positive versus HCV-negative BT patients.
  • To investigate associations between common gene polymorphisms (IL10, TLR7, IL4, IFNG) and allergic status in BT patients.
  • To explore the influence of HCV infection on immune polarization and allergic outcomes in pediatric BT.

Main Methods:

  • Cross-sectional observational study of 46 BT patients (37 HCV-positive, 9 HCV-negative) and 50 controls.
  • Collected clinical allergy history, total IgE levels via ELISA, and performed skin prick tests (SPT) for aeroallergens.
  • Genotyped polymorphisms in IL10, TLR7, IL4, and IFNG genes; analyzed associations descriptively due to small sample size.

Main Results:

  • HCV-positive BT patients had significantly lower mean IgE levels (18.73 IU/mL) and fewer allergic symptoms (21.6%) than HCV-negative patients (118.76 IU/mL; 55.5%).
  • IL10 -1082 AA and TLR7 rs179008 TT genotypes were more prevalent in HCV-positive patients and linked to lower IgE.
  • No significant associations found for IL4 or IFNG variants; splenectomy potentially modified IgE in HCV-negative patients.

Conclusions:

  • Chronic HCV infection may induce immune tolerance, reducing allergic expression in pediatric BT patients.
  • IL10 and TLR7 genotypes may modulate this immune tolerance and allergic phenotype.
  • Larger studies with functional immune profiling are necessary to confirm these exploratory findings.