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Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Comparative Tumor Microenvironment Analysis for HCC and PDAC Using KMplotter.

Wen-Han Chang1, Drashya Shah2, Scott Myers1

  • 1Oncotelic Therapeutics, 29397 Agoura Road, Suite 107, Agoura Hills, CA 91301, USA.

International Journal of Molecular Sciences
|December 30, 2025
PubMed
Summary
This summary is machine-generated.

DNA methyltransferase (DNMT3A) and guanine monophosphate synthetase (GMPS) show context-dependent roles in liver and pancreatic cancers. Their expression impacts patient survival and tumor microenvironments, offering new biomarker potential.

Keywords:
DNMT3AGMPShepatocellular carcinomapancreatic ductal adenocarcinomatoll-like receptor

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Area of Science:

  • Oncology
  • Epigenetics
  • Cancer Metabolism

Background:

  • Hepatocellular carcinoma (HCC) and pancreatic ductal adenocarcinoma (PDAC) are lethal cancers with significant epigenetic and metabolic alterations.
  • DNA methyltransferase (DNMT3A) and guanine monophosphate synthetase (GMPS) are potential prognostic biomarkers, but their roles in different cancer types are not fully understood.

Purpose of the Study:

  • To investigate the prognostic significance and tumor microenvironment associations of DNMT3A and GMPS across hepatocellular carcinoma and pancreatic ductal adenocarcinoma.
  • To explore the potential of DNMT3A and GMPS as context-dependent biomarkers for patient stratification.

Main Methods:

  • Utilized KMplotter for pan-cancer transcriptomic and survival data analysis of over 7000 patients.
  • Performed expression profiling of normal, tumor, and metastatic tissues.
  • Integrated tumor microenvironment (TME) analyses, including immune deconvolution and toll-like receptor signature assessment.

Main Results:

  • Elevated DNMT3A and GMPS correlated with worse overall survival in HCC, especially in Asian patients.
  • In PDAC, high DNMT3A and low GMPS expression predicted favorable outcomes.
  • Both genes were upregulated in tumors versus normal tissues, with DNMT3A linked to immunosuppressive TME niches and GMPS associated with high mutational burden or stromal enrichment.

Conclusions:

  • DNMT3A and GMPS act as context-dependent biomarkers in HCC and PDAC, integrating metabolic and immune signals to influence prognosis.
  • Findings provide mechanistic insights into cancer progression and suggest translational relevance for patient stratification strategies.