Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assessment of Diffusion and Perfusion01:17

Assessment of Diffusion and Perfusion

1.5K
Understanding and evaluating diffusion and perfusion is critical in assessing a patient's respiratory and circulatory health. These processes play key roles in maintaining the body's internal environment, ensuring that tissues receive adequate oxygen while waste products are efficiently removed.
The Role of Diffusion in Respiration
Diffusion is the process by which molecules move from an area of higher concentration to an area of lower concentration. In the respiratory system, this...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of Klhl14 in senescence and epithelial-to-mesenchymal transition via TGF-β modulation.

Cell death & disease·2026
Same author

Image-guided activation of drugs with electromagnetic radiation.

Nature chemical biology·2026
Same author

Margin status after breast-conserving surgery for breast cancer in the Netherlands from 2009 to 2022.

BJS open·2026
Same author

Near-Infrared Fluorescence Imaging for Intraoperative Perfusion Assessment of the Bone During Elective Non-union Surgery: A Feasibility Study.

JB & JS open access·2026
Same author

Effect of Healthy and Tumor-Associated Breast Adipose Tissue on Breast Cancer Cell Migration and Activation.

Cancers·2026
Same author

Prediction of distant metastases at diagnosis in medullary thyroid cancer: combining calcitonin with lymph node imaging.

Journal of the Endocrine Society·2026

Related Experiment Video

Updated: Jan 7, 2026

Observational Study Protocol for Repeated Clinical Examination and Critical Care Ultrasonography Within the Simple Intensive Care Studies
10:38

Observational Study Protocol for Repeated Clinical Examination and Critical Care Ultrasonography Within the Simple Intensive Care Studies

Published on: January 16, 2019

20.7K

Standardized and Quantitative ICG Perfusion Assessment: Feasibility and Reproducibility in a Multicentre Setting.

Eline Feitsma1, Hugo Schouw1,2,3,4, Tim Hoffman1,5,6,7

  • 1Department of Surgery, University Medical Centre Groningen, 9713 GZ Groningen, The Netherlands.

Life (Basel, Switzerland)
|December 30, 2025
PubMed
Summary
This summary is machine-generated.

Indocyanine green near-infrared fluorescence (ICG-NIRF) imaging shows variability in assessing parathyroid function after thyroidectomy. Standardizing ICG concentration and injection is crucial for the Workflow model for ICG-angiography integrating Standardization and Quantification (WISQ) to improve clinical utility.

Keywords:
ICG AngiographyNear-Infrared Fluorescencehypoparathyroidismindocyanine greenparathyroid perfusionperfusion quantificationstandardizationthyroidectomy

More Related Videos

Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling
12:29

Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling

Published on: May 30, 2011

14.2K
Author Spotlight: Advancing Eye Physiology Research via a Multi-Channel Flow Culture for Optimal Tissue Maintenance and Real-Time Assessment
06:26

Author Spotlight: Advancing Eye Physiology Research via a Multi-Channel Flow Culture for Optimal Tissue Maintenance and Real-Time Assessment

Published on: July 14, 2023

1.7K

Related Experiment Videos

Last Updated: Jan 7, 2026

Observational Study Protocol for Repeated Clinical Examination and Critical Care Ultrasonography Within the Simple Intensive Care Studies
10:38

Observational Study Protocol for Repeated Clinical Examination and Critical Care Ultrasonography Within the Simple Intensive Care Studies

Published on: January 16, 2019

20.7K
Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling
12:29

Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling

Published on: May 30, 2011

14.2K
Author Spotlight: Advancing Eye Physiology Research via a Multi-Channel Flow Culture for Optimal Tissue Maintenance and Real-Time Assessment
06:26

Author Spotlight: Advancing Eye Physiology Research via a Multi-Channel Flow Culture for Optimal Tissue Maintenance and Real-Time Assessment

Published on: July 14, 2023

1.7K

Area of Science:

  • Surgical Innovation
  • Medical Imaging
  • Endocrinology

Background:

  • Indocyanine green near-infrared fluorescence (ICG-NIRF) imaging is used for tissue perfusion assessment.
  • Subjective interpretation of ICG-NIRF limits correlation with postoperative parathyroid function.
  • The Workflow model for ICG-angiography integrating Standardization and Quantification (WISQ) was developed to address these limitations.

Purpose of the Study:

  • To evaluate the reproducibility of the WISQ model in adults undergoing total thyroidectomy.
  • To assess the potential of WISQ parameters in predicting postoperative hypoparathyroidism (HPT).

Main Methods:

  • Prospective multicenter study at two Dutch university centers.
  • Standardized intraoperative ICG-NIRF imaging with blood volume-adjusted dosing.
  • Analysis of perfusion curves using predefined regions of interest.

Main Results:

  • Significant inter-center variability in maximum fluorescence intensity, inflow, and outflow slopes was observed.
  • No WISQ parameter significantly predicted postoperative HPT.
  • Reproducible perfusion curves required freshly dissolved ICG (0.5 mg/mL) and consistent injection techniques.

Conclusions:

  • ICG concentration and injection technique significantly influence perfusion kinetics.
  • The WISQ model requires updates, particularly regarding standardized injection dilution, to enhance clinical utility.
  • Further standardization is needed for reliable ICG-NIRF assessment of parathyroid function.