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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Correction: Yalçın et al. Impact of SGLT2 Inhibitors on Cardiovascular Risk Scores, Metabolic Parameters, and Laboratory Profiles in Type 2 Diabetes. <i>Life</i> 2025, <i>15</i>, 722.

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Related Experiment Video

Updated: Jan 7, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

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Advances in Quantitative Techniques for Mapping RNA Modifications.

Ling Tian1, Bharathi Vallabhaneni1, Yie-Hwa Chang2

  • 1Mediomics, LLC, 5445 Highland Park Drive, Saint Louis, MO 63110, USA.

Life (Basel, Switzerland)
|December 30, 2025
PubMed
Summary
This summary is machine-generated.

RNA modifications regulate gene expression and cellular functions. This review details methods for analyzing these crucial RNA modifications, aiding disease biomarker discovery and therapeutic development.

Keywords:
RNA modificationsbiomarker discoverychemical-assisted sequencingepitranscriptomicsmass spectrometrynanopore direct RNA sequencingsingle-cell analysissingle-molecule imaging

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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • RNA modifications are vital regulators of gene expression and cellular functions, impacting RNA stability, splicing, translation, and localization.
  • Their dysregulation is implicated in various diseases, including cancer, neurodegenerative disorders, and viral infections.
  • Accurate quantification and mapping of RNA modifications are essential for elucidating their biological roles.

Purpose of the Study:

  • To review and summarize current and emerging methodologies for analyzing RNA modifications.
  • To discuss the advantages, limitations, and challenges of each analytical technique.
  • To provide a framework for selecting appropriate RNA modification analysis strategies.

Main Methods:

  • Mass spectrometry
  • Antibody-based and non-antibody-based detection methods
  • PCR- and NMR-based detection
  • Chemical- and enzyme-assisted sequencing
  • Nanopore direct RNA sequencing
  • Single-cell and single-molecule imaging techniques

Main Results:

  • A comprehensive overview of diverse RNA modification analysis techniques is presented.
  • The review critically evaluates the strengths and weaknesses of each method.
  • Advanced techniques for single-cell and single-molecule analysis are highlighted.

Conclusions:

  • A robust toolkit for epitranscriptome analysis is available, encompassing various technologies.
  • These methods are crucial for understanding RNA modification functions and identifying disease biomarkers.
  • Future research should focus on high-throughput, multiplexed, and single-cell approaches for comprehensive epitranscriptome decoding.