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Related Experiment Video

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Integrated Transcriptomic Analysis Leveraging Single-Cell and Bulk RNA Sequencing Data to Uncover Pyroptosis-Related

Jiangbo Li1, Yupeng Di2, Xiaoli Kang2

  • 1Bioinformatics Center of AMMS, Beijing, People's Republic of China.

Journal of Hepatocellular Carcinoma
|December 30, 2025
PubMed
Summary

This study developed a prognostic signature for hepatocellular carcinoma (HCC) using pyroptosis-related genes. A high-risk score indicates a poor prognosis and is linked to immunosuppressive features in HCC tumors.

Keywords:
HNRNPA2B1hepatocellular carcinomaprognosispyroptosissingle-cell RNA sequencing

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Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Pyroptosis's role in tumors is complex and not fully understood, especially in hepatocellular carcinoma (HCC).
  • Developing accurate prognostic tools for HCC is crucial for patient outcomes.

Purpose of the Study:

  • To develop a prognostic signature for HCC based on pyroptosis-related genes (PRGs).
  • To identify key PRGs that can predict HCC patient prognosis and understand their role in the tumor microenvironment.

Main Methods:

  • Integrated single-cell RNA sequencing (scRNA-seq) and bulk RNA-seq data from HCC patients.
  • Utilized Weighted Co-Expression Network Analysis and LASSO regression to identify hub PRGs and construct a prognostic model.
  • Analyzed biological functions, survival, immune characteristics, and therapy response associated with the prognostic signature.

Main Results:

  • Identified five key PRGs (ADGRE2, FCER1G, SLC9A9, CYBB, SLAMF6) forming a prognostic signature for HCC.
  • Established a risk score based on these PRGs, which independently predicts HCC prognosis.
  • Found that a high-risk score correlates with poor prognosis and immunosuppressive features within the HCC tumor microenvironment.

Conclusions:

  • This study uniquely integrates single-cell and bulk transcriptomic data to systematically identify pyroptosis-related prognostic biomarkers.
  • Pinpointed the cellular origin of these biomarkers within the HCC tumor microenvironment.
  • The developed prognostic signature offers a novel tool for predicting HCC outcomes and understanding the role of pyroptosis.