Related Experiment Video
Updated: Jan 7, 2026
In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
Published on: January 9, 2020
KUSCC-192: A Novel Tonsillar Squamous Cell Carcinoma Cell Line With Mixed (Episomal and Integrated)-Typed Human
Jae Hyeok Kim1, Jung Hwa Moon1, Cha Yeon Kim1
1Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute of Medical Science, Konkuk University School of Medicine, Seoul, Republic of Korea.
Background:
Human papillomavirus-positive (HPV+) head and neck squamous cell carcinoma (HNSCC) generally has a better prognosis, but some cases are aggressive with poor outcomes. This study established KUSCC-192, a de novo HPV+ tonsillar cancer cell line, and evaluated its tumorigenicity.
Method:
The HPV status in KUSCC-192 was assessed using RT-qPCR, RT-PCR, and FISH. Aggressive characteristics were compared with other HPV+ cell lines and HPV- cell lines.
Result:
KUSCC-192 exhibited a mixed type with a predominantly diffused FISH pattern and a few dotted signals and lower E2 gene expression. These findings, combined with its aggressive features, suggest that the mixed type may contribute to the poor prognosis observed in 2%-30% of HPV+ HNSCC cases.
Conclusion:
KUSCC-192 is a valuable model to study aggressive HPV+ cancer behaviors. Further research is needed to explore the clinical significance of mixed-type HPV integration in HNSCC.
