VAMP5 promotes glioma progression through bioinformatics analysis clinical correlation and functional validation

Area of Science:

• Neuro-oncology
• Molecular Biology
• Cancer Genomics

Background:

• Glioma is an aggressive brain tumor with poor prognosis.
• Novel molecular drivers are needed for improved glioma diagnosis and treatment.
• Vesicle-associated membrane protein 5 (VAMP5) role in glioma is investigated.

Purpose of the Study:

• Investigate VAMP5 expression pattern in glioma.
• Determine the clinical significance and functional role of VAMP5.
• Explore VAMP5 as a potential biomarker and therapeutic target.

Main Methods:

• Integrated TCGA and GEO datasets for VAMP5 expression analysis.
• Validated VAMP5 expression using Real-time PCR, immunohistochemistry, and Western blot.
• Conducted in vitro and in vivo experiments to elucidate VAMP5 function.

Main Results:

• VAMP5 was significantly upregulated in glioma tissues.
• VAMP5 expression correlated with immune cell infiltration and adverse clinical outcomes.
• VAMP5 was found to promote glioma cell proliferation and migration.

Conclusions:

• VAMP5 promotes glioma progression and is linked to immune modulation.
• VAMP5 may serve as a prognostic biomarker for glioma.
• VAMP5 represents a potential therapeutic target for glioma treatment.