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Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
Published on: December 27, 2010
Identification of a Novel HIV-1 Circulating Recombinant Form (CRF184_BC) Among Heterosexual Populations Along the
Min Chen1,2,3, Huichao Chen4, Yanling Ma4
1Yunnan International Joint Laboratory of Public Health and Disease Control, Yunnan Center for Disease Control and Prevention, Kunming, Yunnan, China.
Abstract:
The China-Myanmar border region is recognized as a hotspot for the emergence of HIV-1 recombinant forms. This study identified a novel HIV-1 circulating recombinant form (CRF) in this area, designated CRF184_BC. Near-full-length genome sequences were obtained from four individuals infected through heterosexual contact: one Chinese individual and three Burmese individuals. These sequences formed a distinct monophyletic clade with strong bootstrap support, separate from all known subtypes/CRFs. Recombination analysis revealed a conserved mosaic structure comprising four subtype C segments and three subtype B segments: IC (790-2897), IIB (2898-3208), IIIC (3209-5983), IVB (5984-6435), VC (6436-8821), VIB (8822-8969), and VIIC (8970-9468). Bayesian Markov chain Monte Carlo analysis indicated that CRF184_BC emerged between the mid-2000s and the early 2010s. Identifying emerging CRFs highlights the ongoing dynamic evolution of HIV-1 in this region and emphasizes the need for enhanced molecular surveillance to inform effective public health strategies and vaccine development efforts.
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