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Updated: Jun 30, 2026

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
Published on: December 16, 2016
Unlocking accurate microplastic data: Advanced pump systems for diverse aquatic environments
Thao Thanh Le1, Hoa Thi Tran2, Van Thuong Truong3
1Environmental Chemistry and Ecotoxicology, Phenikaa School of Engineering, Phenikaa University, Hanoi 12116, Viet Nam; Faculty of Biotechnology, Chemistry and Environmental Engineering, Phenikaa School of Engineering, Phenikaa University, Hanoi 12116, Viet Nam.
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Microplastics (MiPs) pose a significant environmental threat due to their persistence and potential toxicity to biota that interact with or ingest them. Accurate assessment of MiP concentrations in aquatic systems is critical for understanding their distribution and impacts. This study develops the MiPCS Pump (Microplastic Collector System), an innovative pump-based sampling system integrating 80 μm mesh plankton nets. The system was tested against traditional net-based methods (manta and Apstein nets) in the Red River Delta and Cat Ba Archipelago, Vietnam. This innovative design features a suction basket excluding large particles (>5 mm), a mixing chamber and 5-way splitter for uniform distribution, and precise flow control (via flow metering) to five phytoplankton nets, ensuring accurate and simultaneous multi-sample collection. This advanced system provided more consistent and precise results, sampling a broader spectrum of MiP sizes, and enabling sampling across a variety of water depths and environmental conditions. Quantitatively, MiPCS Pump yielded significantly higher MiP concentrations (2.95-7.80 mg m-3 and 16.25-114.58 particles m-3) compared to the net methods (1.59-3.27 mg m-3 and 0.78-26.62 particles m-3). Although nets are effective for larger surface MiPs (mesoplastics) there is a lack of understanding of MiP catchability in nets, whereas the MiPCS Pump offers precise volume measurement, adaptability to various depths, and captures a broader size range, making it a more comprehensive method for assessing MiP pollution. This MiPCS Pump system is crucial for ensuring accurate and comparable data for global MiP monitoring and the development of effective plastic waste management policies.

