Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Calcium Phosphate Mineralization of Bulk Alginate Hydrogels Composites With Metal and Metal Oxide Nanoparticles.

Macromolecular bioscience·2026
Same author

An organotypic model of ductular reaction reveals a mevalonate-dependent vulnerability in reactive biliary cells.

Cell reports·2025
Same author

Thermally Cured Gelatin-Methacryloyl Hydrogels Form Mechanically Modulating Platforms for Cell Studies.

Biomacromolecules·2025
Same author

Microenvironmental control of the ductular reaction: balancing repair and disease progression.

Cell death & disease·2025
Same author

Evaluation of the effect of enzymatic pretreatment with l-asparaginase on acrylamide formation during microwave, air, and deep frying of potatoes.

Journal of food science·2025
Same author

Green technologies for extracting plant waste functional ingredients and new food formulation: A review.

Journal of food science·2024

Related Experiment Video

Updated: Jan 7, 2026

Generation of Organoids from Mouse Extrahepatic Bile Ducts
09:13

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Published on: April 23, 2019

10.5K

Next-Generation Hydrogels for Biliary Organoid Engineering.

Andrea Marfoglia1,2, Giovanni Sorrentino1,2

  • 1Department of Medical, Surgical and Health Sciences, University of Trieste, Strada di Fiume 477, 34139 Trieste, Italy.

Pharmaceuticals (Basel, Switzerland)
|December 31, 2025
PubMed
Summary

Biliary organoids offer a promising model for liver diseases. Engineered hydrogels provide a defined, tunable environment for organoid culture, advancing disease modeling and potential therapies.

Keywords:
bile ductsbiliary organoidsbiomaterialsbiomechanicscholangiopathiesdisease modelshydrogelsliverregenerative medicinetissue engineering

More Related Videos

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
08:34

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

17.3K
Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts
09:55

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Published on: May 16, 2020

4.1K

Related Experiment Videos

Last Updated: Jan 7, 2026

Generation of Organoids from Mouse Extrahepatic Bile Ducts
09:13

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Published on: April 23, 2019

10.5K
Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
08:34

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

17.3K
Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts
09:55

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Published on: May 16, 2020

4.1K

Area of Science:

  • Bioengineering
  • Hepatology
  • Biomaterials Science

Background:

  • Cholangiopathies, or biliary disorders, significantly impair liver function and often necessitate liver transplantation.
  • Current bioengineered strategies are needed to study cholangiopathies and develop regenerative therapies.
  • Biliary organoids provide a physiologically accurate in vitro model for liver diseases.

Purpose of the Study:

  • To review advances in biliary organoid bioengineering.
  • To discuss the role of engineered hydrogels in improving biliary organoid platforms.
  • To highlight the potential of hydrogel-based systems for disease modeling and clinical translation.

Main Methods:

  • Review of current literature on biliary organoid culture and bioengineering.
  • Analysis of basement membrane extract (BME) limitations.
  • Exploration of engineered hydrogel properties and applications in organoid culture.

Main Results:

  • Biliary organoids offer enhanced physiological relevance, patient specificity, and scalability compared to traditional models.
  • Basement membrane extract (BME) matrices have undefined composition and animal origin, limiting reproducibility and clinical translation.
  • Engineered hydrogels offer chemically defined, tunable microenvironments for precise control over organoid culture.

Conclusions:

  • Engineered hydrogels represent a significant advancement for biliary organoid culture, overcoming limitations of traditional matrices.
  • Hydrogel-based platforms are crucial for developing next-generation biliary models for disease study and therapeutic development.
  • Further development of these systems holds promise for more translationally relevant biliary models.