Antioxidant and Anticancer Activities of Water Extracts from Flowers, Leaves and Stems of In Vitro Cultivated and Wild-Growing Marrubium vulgare Plants
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Summary
This summary is machine-generated.Marrubium vulgare propagation was optimized, yielding extracts with significant antioxidant and anticancer properties. Both wild and cultivated plants show potential for natural therapeutics and cytoprotective formulations.
Area Of Science
- Plant Biotechnology
- Pharmacognosy
- Cell Biology
Background
- Marrubium vulgare (horehound) possesses diverse pharmacological potential but lacks efficient in vitro cultivation and studied biological activities of its extracts.
- Limited research exists on in vitro propagation and comparative biological potential of extracts from different M. vulgare parts.
Purpose Of The Study
- To develop an efficient in vitro propagation protocol for M. vulgare.
- To compare antioxidant and anticancer activities of aqueous extracts from in vitro cultivated and wild-growing M. vulgare parts.
Main Methods
- Micropropagation using Murashige and Skoog medium with kinetin and indole-3-acetic acid.
- Quantification of total polyphenols and flavonoids.
- Assay of antioxidant capacity (ORAC, HORAC).
- Evaluation of antiproliferative effects against HeLa, HT-29, and MCF-7 cancer cell lines.
- Apoptosis and cell cycle analysis to determine cytotoxicity mechanisms.
Main Results
- Achieved 90% shoot formation efficiency in vitro.
- Cultivated M. vulgare leaves exhibited highest polyphenol, flavonoid content, and antioxidant activity.
- Aqueous extracts, especially from leaves and flowers, showed selective antiproliferative effects, with HeLa cells being most sensitive.
- Extracts induced apoptosis and cell cycle arrest (G1 for cultivated, G1/G2/M for wild plants).
Conclusions
- An efficient micropropagation protocol for M. vulgare was established, ensuring a sustainable source of bioactive material.
- This study offers the first comprehensive comparison of M. vulgare extracts from in vitro and wild sources, linking phytochemicals to bioactivity.
- Both wild and cultivated M. vulgare are promising for natural anticancer therapeutics, with cultivated plants showing potential for antioxidant and cytoprotective formulations.
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