Related Experiment Video

Updated: Jun 2, 2026

Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA
12:18

Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA

Published on: February 9, 2011

23.6K

Characterization and Pathogenicity of Mannheimia glucosida Isolated from Sheep

Qibing Gu1, Min Gao1, Taichun Gao1

  • 1College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China.

Microorganisms
|December 31, 2025
PubMed

Insights

Mannheimia glucosida causes respiratory disease in sheep and damages multiple organs in mice. This study identifies key virulence genes and establishes a PCR assay for diagnosing this important sheep pathogen in China.

Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Ruminant Health

Background:

  • Bacteria of the genus Mannheimia are significant pathogens causing respiratory diseases in ruminants.
  • The biological characteristics and pathogenic mechanisms of Mannheimia glucosida are not well understood.
  • Mannheimia glucosida poses a threat to the global ruminant industry.

Purpose of the Study:

  • To isolate and characterize Mannheimia glucosida strains from sheep with respiratory disease in China.
  • To investigate the virulence factors and pathogenic mechanisms of M. glucosida.
  • To develop a diagnostic tool for M. glucosida.

Main Methods:

  • Isolation and identification of M. glucosida from diseased sheep.
  • Virulence gene assay, phylogenetic analysis (16S rRNA, infB, sodA), and mouse modeling.
  • Whole genome sequencing, bioinformatic analysis, and PCR assay development.

Main Results:

  • Five strains of M. glucosida were isolated, all hydrolyzing esculin, encoding adh and plpD virulence genes, and resistant to streptomycin.
  • Phylogenetic analysis suggested sodA as a valuable marker for bacterial evolution in Mannheimia.
  • Mouse model showed M. glucosida D251 caused multiorgan damage (LD50 = 1.35 × 10^6 CFU) and possessed numerous virulence and drug resistance genes.
  • A sensitive and specific PCR assay for M. glucosida was successfully established.

Conclusions:

  • M. glucosida is identified as a potential significant pathogen contributing to respiratory disease in Chinese sheep.
  • The study provides insights into the virulence factors and genetic characteristics of M. glucosida.
  • The developed PCR assay offers a valuable tool for clinical diagnosis and treatment of M. glucosida infections in sheep.
Keywords:
Mannheimia glucosidabiochemical characterizationdrug resistancerespiratory diseasesvirulence

More Related Videos

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model07:26

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model

Published on: July 1, 2018

7.7K
Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery10:03

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

Published on: November 5, 2019

7.6K

Related Concept Videos

Determinants of Bacterial Pathogenicity and Virulence01:20

Determinants of Bacterial Pathogenicity and Virulence

Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...
Bacterial Meningitis01:24

Bacterial Meningitis

Bacterial meningitis is a severe infectious disease involving inflammation of the meninges, the protective membranes surrounding the brain and spinal cord. It occurs when pathogenic bacteria cross the blood–brain barrier and enter the cerebrospinal fluid. Common causative organisms include Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae type b, Listeria monocytogenes, and Escherichia coli K1. The exact route of entry varies by pathogen and host condition.Routes of Entry...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Bacterial Meningitis II: Pathophysiology01:26

Bacterial Meningitis II: Pathophysiology

Bacterial meningitis typically begins when pathogens such as Neisseria meningitidis and Streptococcus pneumoniae colonize the nasopharynx and invade the bloodstream. This process is facilitated by bacterial virulence factors, such as polysaccharide capsules, which resist phagocytosis and complement-mediated killing. Less commonly, bacteria reach the central nervous system via contiguous spread from infections like otitis media or sinusitis, through congenital or acquired dural defects, or...