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Updated: Jan 7, 2026

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates
Published on: July 2, 2018
LuxR-Type Regulator RRP6 Positively Regulates the Biosynthesis of Plantaricin EF and Improves Its Production in
Yaxuan Liu1, Siqi Liu1, Zixian Li1
1School of Life Science, Huaibei Normal University, Huaibei 235000, China.
Abstract:
The two-component system HPK6/RRP6 related to the pln locus of plantaricin biosynthesis was screened out. The overexpression of LuxR-type regulator RRP6 promoted the transcription of ABC transporter-related genes, thereby increasing plantaricin EF yield. Its yield in 163(rrp6) reached 16.01 mg/L, which was 1.20-fold that of the original strain. The regulatory mechanism indicated that RRP6 could bind to two sites of the plnG1 promoter, promoting its transcription and translation, accelerating the secretion of plantaricin and auto-inducing peptide, and enhancing the extracellular plantaricin yield. Amino acids Q73, R144, T171, and Y175 play a crucial role in the binding of RRP6. Furthermore, potential regulatory compensation within the Lactiplantibacillus plantarum 163 genome may compensate for the negative effects after the deletion of rrp6. These results provide a novel strategy for increasing plantaricin EF yield, which facilitates its large-scale application as a natural and safe food preservative in agriculture and the food industry.
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