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Epstein-Barr Virus Promotes Gastric Cancer Progression by Modulating m6A-Dependent YTHDF1-TSC22D1 Axis.

Yea Rim An1,2, Jaehun Jung3, Kyeong Min Kwon1,2

  • 1Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.

Microorganisms
|December 31, 2025
PubMed
Summary
This summary is machine-generated.

Epstein-Barr virus (EBV) infection reduces m6A methylation of TSC22D1 in gastric cancer. The YTHDF1 protein degrades TSC22D1 mRNA, offering a potential therapeutic target for EBV-associated gastric cancer.

Keywords:
Epstein–Barr virusTSC22D1YTHDF1epitranscriptomicsgastric cancerm6A modification

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Area of Science:

  • Molecular biology
  • Oncology
  • Virology

Background:

  • Epstein-Barr virus (EBV) is linked to gastric cancer.
  • The role of EBV in m6A-dependent gene regulation in gastric cancer is unclear.
  • N6-methyladenosine (m6A) modification impacts gene expression and stability.

Purpose of the Study:

  • To investigate how EBV infection affects m6A methylation patterns in gastric cancer cells.
  • To examine the impact of EBV on TSC22D1 mRNA stability via the YTHDF1 protein.
  • To identify potential therapeutic targets in EBV-associated gastric cancer.

Main Methods:

  • m6A RNA immunoprecipitation sequencing (MeRIP-seq) was used to analyze m6A methylation.
  • Gastric cancer cell lines (AGS) with and without EBV infection (AGS-EBV) were compared.
  • YTHDF1 knockdown was performed to assess its effect on TSC22D1.

Main Results:

  • EBV infection significantly reduced m6A methylation of TSC22D1 in AGS-EBV cells compared to AGS cells.
  • YTHDF1 knockdown led to increased stability and expression of TSC22D1 mRNA.
  • YTHDF1 was shown to bind TSC22D1 mRNA, promoting its m6A-dependent degradation.

Conclusions:

  • EBV infection alters m6A modification in gastric cancer cells.
  • The YTHDF1-TSC22D1 interaction regulates gene stability in an m6A-dependent manner.
  • The YTHDF1-TSC22D1 axis represents a potential therapeutic strategy for EBV-associated gastric cancer.