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Genomic Characterization of a Class 2/1 Hybrid Integron-Driven Multidrug-Resistant Proteus mirabilis SH-8 of Swine
Xinyu Wang1, Hui Su1, Nuo Xu1
1College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin 132101, China.
Abstract:
Objective:P. mirabilis is a Gram-negative rod-shaped bacterium. In this study, the SH-8 strain of P. mirabilis was isolated from swine-derived samples, and its antibiotic resistance genes (ARGs) and transmission mechanisms were systematically characterized. Methods: The minimum inhibitory concentrations (MICs) of strain SH-8 were determined by the broth microdilution method, with genomic features analyzed through whole-genome sequencing (WGS). Results:P. mirabilis SH-8 exhibited resistance to multiple antimicrobial agents. Its genome consists of a single chromosome (3,993,987 bp; GC content 38.8%) and one plasmid. WGS identified 21 distinct ARGs in this strain, along with a class 2/1 hybrid integron. Furthermore, a heatmap analysis of resistance genes from 29 P. mirabilis isolates of diverse origins revealed a striking similarity between the resistance gene profile of SH-8 and those of other strains. Conclusions: The MDR exhibited by P. mirabilis SH-8 is primarily attributed to integron-mediated resistance gene clusters within its genome, which may pose challenges for treating opportunistic infections caused by this bacterium.
Insights
This study characterized the SH-8 strain of Proteus mirabilis, revealing multiple antibiotic resistance genes (ARGs) and a hybrid integron. This multidrug resistance (MDR) in P. mirabilis poses challenges for treating opportunistic infections.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Proteus mirabilis is a Gram-negative bacterium.
- This study focused on the SH-8 strain isolated from swine samples.
Purpose of the Study:
- To characterize antibiotic resistance genes (ARGs) and transmission mechanisms in P. mirabilis SH-8.
- To investigate the genomic features contributing to multidrug resistance (MDR).
Main Methods:
- Minimum inhibitory concentrations (MICs) determined by broth microdilution.
- Whole-genome sequencing (WGS) for genomic analysis.
- Heatmap analysis of resistance genes from diverse P. mirabilis isolates.
Main Results:
- P. mirabilis SH-8 exhibited resistance to multiple antimicrobial agents.
- WGS identified 21 distinct ARGs and a class 2/1 hybrid integron.
- SH-8's resistance gene profile showed similarity to other P. mirabilis strains.
Conclusions:
- MDR in P. mirabilis SH-8 is linked to integron-mediated resistance gene clusters.
- This resistance poses challenges for treating opportunistic infections.
- Understanding these mechanisms is crucial for effective antimicrobial strategies.
