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In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

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Let-7f-5p Inhibits PRRSV Replication by Regulating Lipid Metabolic Reprogramming in Infected Cells.

Dongfeng Jiang^1,2, Jin Huang¹, Xiaotong Wang¹

¹College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.

Veterinary Sciences

|December 31, 2025

Summary

This summary is machine-generated.

Porcine reproductive and respiratory syndrome virus (PRRSV) manipulates host lipid metabolism for replication. The microRNA let-7f-5p inhibits PRRSV by reducing lipid accumulation and suppressing viral protein expression, offering a potential antiviral strategy.

Keywords:

PRRSV SREBP2 let-7f-5p lipid metabolism pig

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Area of Science:

Virology
Molecular Biology
Biochemistry

Background:

Lipids are crucial for viral replication and infection.
The interplay between lipid metabolism and PRRSV proliferation is not well understood.

Purpose of the Study:

To investigate the impact of PRRSV infection on host lipid metabolism.
To explore the role of let-7f-5p in modulating lipid metabolism and PRRSV replication.

Main Methods:

Transcriptomics, lipidomics, BODIPY staining, Western blot (WB), and quantitative reverse transcription PCR (qRT-PCR).
Analysis of lipid metabolism-associated genes and metabolites in PRRSV-infected cells.
Investigation of let-7f-5p effects on lipid droplets and viral protein expression.

Main Results:

PRRSV infection altered lipid metabolism genes and metabolites, increasing intracellular lipid droplets and upregulating SREBP2.
Let-7f-5p reduced lipid droplet accumulation and suppressed PRRSV N protein expression.
Fifteen upregulated lipid species were downregulated by let-7f-5p, impacting phosphatidylcholine and fatty acid metabolism pathways.

Conclusions:

PRRSV hijacks host lipid metabolism to promote viral replication.
Let-7f-5p exhibits antiviral effects against PRRSV through dual mechanisms, targeting lipid metabolism and viral protein synthesis.
Modulating host lipid metabolism presents a potential host-directed antiviral strategy against PRRSV.