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Role of EpCAM-Positive Exosomes in Ovarian Cancer: MiRNA Signatures of Chemoresistance and Disease Progression.

Samruddhi Ranmale¹, Pavan Kumar¹, Hemant Tongaonkar²

¹ICMR-National Institute for Research in Reproductive and Child Health, Mumbai, India.

Asia-Pacific Journal of Clinical Oncology

|December 31, 2025

Summary

Ovarian cancer (OC) patients show elevated levels of specific exosomal microRNAs (miRNAs) linked to chemoresistance and metastasis. EpCAM-positive exosomes may serve as biomarkers for monitoring OC progression and relapse.

Keywords:

chemotherapy clinical specimens exosomes miRNA ovarian cancer

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Area of Science:

Oncology
Molecular Biology
Biochemistry

Background:

Ovarian cancer (OC) presents diagnostic and chemoresistance challenges.
Exosomes mediate intercellular communication, influencing tumor progression and drug resistance.
Exosomal microRNAs (miRNAs) are implicated in OC's complex biology.

Purpose of the Study:

Investigate the role of specific exosomal miRNAs in OC.
Focus on their involvement in chemoresistance, immune evasion, and metastasis.
Identify potential biomarkers for OC monitoring.

Main Methods:

Real-time PCR was used to measure exosomal and non-exosomal miRNA levels (miR-182, miR-9, miR-130a, miR-20a, miR-23b).
Serum samples were analyzed from 60 women: 20 chemo-naive OC, 20 chemotherapy-treated OC, and 20 healthy controls.
Exosomes were fractionated into EpCAM-positive and EpCAM-negative populations.

Main Results:

Ovarian tumors release more EpCAM-positive exosomes containing specific miRNAs.
Elevated miR-182 and miR-9 were detected in EpCAM-positive exosomes from OC patients.
miR-182 correlated with disease relapse and FIGO stage; miR-20a increased post-chemotherapy.
No significant changes in miRNA expression were observed in EpCAM-negative exosomes.

Conclusions:

EpCAM-positive exosomes likely facilitate miRNA packaging and release in OC.
These exosomes offer a potential mechanism for OC intercellular communication.
Exosomal miRNAs show promise as biomarkers for OC progression and relapse monitoring.