Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

In the mood for flow: mood intervention effects on flow experiences.

Cognition & emotion·2026

Same author

Design of bacterial DNT sensors based on computational models.

Nucleic acids research·2026

Same author

AI-directed gene fusing prolongs the evolutionary half-life of synthetic gene circuits.

Science advances·2025

Same author

The bioinformatics of the finding that the hepatitis delta virus RNA editing mechanism by a conformational switch exists in genotype 7 in addition to genotype 3.

Briefings in bioinformatics·2025

Same author

Translon: a single term for translated regions.

Nature methods·2025

Same author

Deciphering plasmid replication dynamics: a computational approach to predict ColE1-like plasmid copy number.

Journal of the Royal Society, Interface·2025

Same journal

An epigenetic clock for chronological age estimation in East Asian populations.

NAR genomics and bioinformatics·2026

Same journal

The role of ATF4 in neurons under mitochondrial stress.

NAR genomics and bioinformatics·2026

Same journal

Distinct repeat architecture landscapes in the proteomes of protozoan parasites.

NAR genomics and bioinformatics·2026

Same journal

Long non-coding RNA triplex-dependent regulation of melanoma gene networks.

NAR genomics and bioinformatics·2026

Same journal

Challenges in predicting chromatin accessibility differences between species.

NAR genomics and bioinformatics·2026

Same journal

Power-law penalties correct distance bias in single-cell co-accessibility and deep-learning chromatin interaction predictions.

NAR genomics and bioinformatics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 7, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

Designing genetically stable multicopy gene constructs with the ChimeraUGEM web server.

Moritz Burghardt¹, Alon Diament¹, Tamir Tuller^1,2,3

¹Department of Biomedical Engineering, The Iby and Aladar Fleischman Faculty of Engineering, Tel Aviv 69978, Israel.

NAR Genomics and Bioinformatics

|December 31, 2025

Summary

This summary is machine-generated.

We developed a new algorithm, multisequence ChimeraMap (MScMap), to design stable, high-expression gene systems. MScMap minimizes genetic instability in multicopy systems by creating diverse DNA sequences, outperforming existing methods.

More Related Videos

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

The MultiBac Protein Complex Production Platform at the EMBL

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Related Experiment Videos

Last Updated: Jan 7, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

The MultiBac Protein Complex Production Platform at the EMBL

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Area of Science:

Biotechnology
Molecular Biology
Bioinformatics

Background:

High-level protein production often requires integrating multiple gene copies into a host organism.
Multicopy gene systems face genetic instability due to homologous recombination between identical DNA sequences.

Purpose of the Study:

To develop an algorithm that designs multiple synonymous coding sequences to minimize recombination risk and maintain high protein expression.
To enhance genetic stability in multicopy gene systems.

Main Methods:

The multisequence ChimeraMap (MScMap) algorithm was developed, extending the ChimeraMap framework.
MScMap selects diverse nucleotide blocks from a host genome and uses heuristics to reduce common substrings, minimizing recombination.
The algorithm was tested against a multi-objective evolutionary algorithm for human protein optimization.

Main Results:

MScMap demonstrated superior performance in genetic stability and predicted expression compared to a multi-objective evolutionary algorithm.
The method significantly reduces computational time for coding sequence optimization.
MScMap was also effective in reducing sequence repeats within single coding sequences.

Conclusions:

MScMap offers an efficient and effective solution for designing genetically stable, high-expression multicopy gene systems.
The algorithm provides a valuable tool for protein engineering and synthetic biology applications.
A web tool is available for optimizing single and multicopy coding sequences.