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Related Experiment Video

Updated: Jan 7, 2026

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Published on: August 8, 2019

Automation workflow for high-throughput arrayed plasmid DNA preparation and quantification.

Chih-Cheng Yang, Aniruddha J Deshpande, Michael Jackson

Biorxiv : the Preprint Server for Biology

|December 31, 2025

Summary

This summary is machine-generated.

Automating plasmid DNA preparation using the Biomek i7 Hybrid workstation significantly improves yield and quality for high-throughput applications like CRISPR screening. This robotic workflow enhances productivity and reduces errors in generating arrayed plasmid DNA libraries.

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Last Updated: Jan 7, 2026

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Area of Science:

Molecular Biology
Biotechnology
Automation Engineering

Background:

High-throughput plasmid DNA library preparation is crucial for applications like CRISPR screening but is often labor-intensive and costly.
Variations in plasmid yield and quality can compromise the suitability of entire libraries for downstream experiments.
Current manual methods are prone to human error and require significant hands-on time.

Purpose of the Study:

To establish an automated workflow for high-throughput plasmid DNA preparation and quantification.
To increase productivity and reduce human intervention in generating arrayed plasmid DNA libraries.
To ensure consistent yield and high quality of plasmid DNA for downstream applications.

Main Methods:

Utilized the Biomek i7 Hybrid automated workstation for robotic liquid handling and peripheral instrument integration.
Employed alkaline lysis and magnetic bead purification for plasmid DNA extraction in a 96-well plate format.
Quantified plasmid DNA using the PicoGreen assay and compared results with NanoDrop UV spectrophotometry.

Main Results:

The automated workflow achieved median and average yields of approximately 9.5 and 10 µg per sample (7.6-8 µg/mL of culture) via PicoGreen assay.
NanoDrop measurements indicated higher yields (median ~25 µg/sample, ~20 µg/mL culture) with over 98% of samples exceeding 10 µg/mL.
Demonstrated a strong positive correlation (R² > 0.8) between PicoGreen and NanoDrop quantification methods.
DNA gel electrophoresis confirmed high plasmid quality across the generated library.

Conclusions:

The developed automated workflow provides a robust, scalable, and cost-effective solution for high-throughput arrayed plasmid library generation.
Automation significantly enhances productivity and consistency in plasmid DNA preparation, mitigating issues associated with manual methods.
This platform is well-suited for demanding applications requiring large numbers of high-quality plasmid DNA samples.