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Updated: Jan 7, 2026
Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
Published on: September 29, 2015
Comparison of the Effects of Hyaluronic Acid and Platelet-Rich Fibrin on Wound Healing
Adalet Çelebi1, Ezgi Eroğlu Çakmakoğlu2, Dilan Aykanat1
1Department of Oral and Maxillofacial Surgery, Mersin University Faculty of Dentistry, Mersin.
Objective:
In this study, the authors compared the effects of local application of platelet-rich fibrin (PRF) and hyaluronic acid (HA) bone and soft tissue on wound healing in rats.
Materials And Methods:
A total of 30 adult male Wistar Albino rats were used in this study. Twenty-four rats were randomly segregated into 3 groups (n=8 each), namely PRF, HA, and the control group. Six rats were used to obtain PRF. Defects ~8 mm in diameter in the frontal region of the rats were created using a trephine burr with an outer diameter of 8 mm. HA gel was applied to the defects in the HA group, whereas PRF was placed in the defects of the PRF group. The defects in the control group were left empty. The rats were sacrificed 14 days after the procedure. The calvarial bones and surrounding soft tissues of the rats were analyzed histopathologically and immunohistochemically.
Results:
In the PRF group, the mean scores for increases in fibrous connective tissue proliferation (P=0.602), osteoblast proliferation (P=0.453), osteoid matrix formation (P=0.380), and osteoid matrix calcification (P=0.084) were consistently higher compared with the HA and control groups although these differences did not reach statistical significance (P>0.05). In addition, osteoprotegerin (OPG) levels were markedly elevated in the PRF group compared with the control and HA groups (P=0.220). Moreover, a higher proportion of samples in the PRF group exhibited elevated RANKL levels compared with the control and HA groups (P=0.183), suggesting an active remodeling state.
Conclusions:
PRF showed a nonsignificant, yet consistent superiority in enhancing both bone and soft tissue healing parameters and HA have a significant impact on bone and soft tissue healing. However, although both agents seem to impact the healing cascade, the superior OPG expression in the PRF group indicates a potentially stronger anti-resorptive and osteogenic effect. However, further studies are required to comprehensively evaluate their long-term relationship with wound healing.
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