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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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A Universal Light-Activated CRISPR-RNA Based on Split Direct Repeat for One-Pot Cas12a Nucleic Acid Detection.

Meng Cheng1, Yixuan Wang1, Weihong Lin1

  • 1Department of Laboratory Medicine, The Key Laboratory of Advanced Interdisciplinary Studies, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, China.

Analytical Chemistry
|December 31, 2025
PubMed
Summary

Researchers developed a universal light-activated CRISPR-RNA system for precise gene editing and diagnostics. This novel approach enables rapid target detection with high sensitivity and specificity, advancing molecular diagnostics.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • CRISPR-Cas Systems

Background:

  • CRISPR-Cas systems offer potential for gene editing and diagnostics.
  • Current photochemical CRISPR control lacks a universal approach.
  • A modular, light-activated CRISPR-RNA design is needed.

Purpose of the Study:

  • To develop a universal, light-activated CRISPR-RNA system for modular applications.
  • To create a light-controlled CRISPR system adaptable for various targets.
  • To establish a one-pot detection system for enhanced molecular diagnostics.

Main Methods:

  • Designed a modular CRISPR-RNA by splitting crRNA into 5' SDR and 3' SDR-Spacer domains.
  • Introduced double-stranded extensions at the cleavage site for functional integrity.
  • Screened light-sensitive caging groups on the 5' SDR for universal application.

Main Results:

  • Developed a novel light-activated CRISPR-RNA system with a universal caged 5' SDR.
  • Established the UniLight-CRISPR system for one-pot RAA-Cas12a detection.
  • Achieved 95.45% sensitivity and 100% specificity in *Mycoplasma pneumoniae* detection.

Conclusions:

  • The universal photo-regulation strategy overcomes limitations in CRISPR diagnostics.
  • The developed system demonstrates high performance comparable to conventional assays.
  • This approach provides a blueprint for adapting Cas enzymes and broad applications in diagnostics and gene editing.