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Published on: June 9, 2014
Dual-readout digital colorimetric assay for zearalenone in corn based on alkaline phosphatase mediated growth of
Shanghong Ji1, Tingtiao Pan1, Weizhen Xu1
1School of Food and Liquor Engineering (School of Wuliangye Baijiu), Sichuan University of Science and Engineering, Sichuan Province, Yibin 644000, China.
Abstract:
Zearalenone (ZEN) poses a substantial threat to global cereal grain security. Herein, a dual-readout biotin-avidin amplified nanozyme-linked immunosorbent assay (BA-ELISA) based on absorbance and blue intensity was established for detecting ZEN residues in corn. The method utilized alkaline phosphatase (ALP), which hydrolyzes ascorbic acid 2-phosphate (AAP) to generate ascorbic acid (AA). The resulting AA reduced K₃[Fe(CN)₆] to K₄[Fe(CN)₆], subsequently reacting with FeCl₃ to form blue Prussian blue nanoparticles (PBNPs). Concurrently, AAP reacted with FeCl₃ to yield a brown AAP-Fe3+ complex, enabling dual-readout detection via PBNPs and AAP-Fe3+. The assay exhibited a linear detection range of 0.625-10 ng/mL, a limit of detection (LOD) of 0.022 ng/mL via absorbance and 0.00447 ng/mL via digital colorimetry, and spiked recovery rates of 104.63 %-118.64 % in corn samples. This dual-readout digital colorimetric detection provided a convenient, sensitive platform for on-site toxin monitoring, offering new perspectives for food safety inspection.
