Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.6K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.6K
CRISPR01:59

CRISPR

57.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Convergent paths to multicellular complexity in photosynthetic eukaryotes.

Current biology : CB·2026
Same author

Moderate to severe negative symptoms predict low risk of symptoms worsening in schizophrenia patients in CATIE.

Schizophrenia research·2026
Same author

PKN is a sex- and species-specific fertilization factor in brown algae.

Current biology : CB·2026
Same author

Latent endogenous giant viruses drive active infection and inheritance in a multicellular algal host.

Nature microbiology·2026
Same author

Evolution of a distinct chromatin regulatory landscape in brown algae.

Nature ecology & evolution·2026
Same author

Flexible paths to multicellularity.

Nature·2026

Related Experiment Video

Updated: Jan 7, 2026

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

10.0K

Efficient CRISPR-Cas genome editing in brown algae.

Cláudia Martinho1, Masakazu Hoshino2, Morgane Raphalen3

  • 1Department of Algal Development and Evolution, Max Planck Institute for Biology, Max-Planck-Ring 5, 72076 Tübingen, Germany; School of Life Sciences, University of Dundee, Division of Plant Sciences at The James Hutton Institute, Errol Road, DD2 5DA Dundee, UK.

Cell Reports Methods
|December 31, 2025
PubMed
Summary
This summary is machine-generated.

Scientists developed a new CRISPR genome editing tool for brown algae, enabling functional studies of their complex evolution and development. This efficient, transgene-free method works across multiple species, including kelps.

Keywords:
CP: biotechnologyCRISPR-CasRNP transfectionbrown algaegenome editing

More Related Videos

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

12.4K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

23.0K

Related Experiment Videos

Last Updated: Jan 7, 2026

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

10.0K
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

12.4K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

23.0K

Area of Science:

  • Marine Biology
  • Evolutionary Biology
  • Genetics

Background:

  • Brown algae are a unique lineage of complex multicellular organisms, distinct from plants and animals.
  • Functional studies in brown algae have been hindered by a lack of efficient genome editing tools.

Purpose of the Study:

  • To develop a robust, high-efficiency, and transgene-free CRISPR-based genome editing platform for brown algae.
  • To enable functional genomics research in ecologically and biotechnologically important brown algal species.

Main Methods:

  • Optimized a polyethylene glycol (PEG)-mediated ribonucleoprotein (RNP) delivery system in Ectocarpus.
  • Demonstrated precise editing of the IMMEDIATEUPRIGHT (IMM) locus to recreate a known mutant phenotype.
  • Utilized APT/2-fluoroadenine (2-FA) selection to enhance editing specificity.

Main Results:

  • Achieved reproducible, transgene-free genome editing across multiple loci without specialized equipment.
  • Successfully recreated the imm mutant phenotype, validating the platform's efficacy.
  • Demonstrated the platform's transferability to other brown algal species, including kelps.

Conclusions:

  • The developed CRISPR platform provides a powerful new tool for functional genomics in brown algae.
  • This advancement will facilitate research into brown algal development, life cycle regulation, and the evolution of multicellularity.
  • Enables deeper investigation into the independent evolution of complex traits in brown algae.