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Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
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Meningioma cell reprogramming and microenvironment interactions underlie brain invasion.

Ayush Aggarwal1,2,3, Mark W Youngblood4, Thiebaud Picart1,5

  • 1Department of Neurological Surgery, University of California San Francisco, San Francisco, California, USA.

Neuro-Oncology
|December 31, 2025
PubMed
Summary

Brain invasion in meningiomas involves molecular changes and interactions between tumor cells and neurons. This study identifies key genes and cellular players at the brain-tumor interface (BTI), revealing mechanisms of meningioma progression.

Keywords:
RNA sequencingbrain invasioncancer neurosciencemeningiomasingle-cell bioinformaticsspatial bioinformatics

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Area of Science:

  • Neuro-oncology
  • Molecular biology
  • Genomics

Background:

  • Meningioma brain invasion complicates surgery and recurrence risk.
  • Molecular mechanisms driving invasion are largely unknown.

Purpose of the Study:

  • To elucidate molecular and cellular features of brain-invasive meningiomas.
  • To investigate functional interactions at the brain-tumor interface (BTI).

Main Methods:

  • Analyzed bulk, single-cell, and spatial RNA sequencing data from meningiomas.
  • Utilized multiplexed immunofluorescence (IF) for validation.
  • Conducted meningioma/neuron co-culture experiments.

Main Results:

  • Identified conserved gene enrichment (TGM2, S100A11, ZYX, PDGFRA) at the BTI.
  • Confirmed macrophage enrichment at the BTI.
  • Observed neuronal hyperexcitability and increased meningioma cell proliferation in co-cultures.

Conclusions:

  • Meningioma brain invasion is characterized by molecular alterations in tumor cells.
  • Functional interactions within the tumor microenvironment contribute to invasion.