Coumarin-1,2,3-Triazole Conjugates as Molecular Scaffolds for the Selective Induction of ROS-Driven Apoptosis in...

The increasing incidence of cancer and the emergence of drug resistance underscore the urgent need for new therapeutic options. This study aimed to synthesize and evaluate new coumarin-triazole hybrids for their cytotoxic selectivity and safety profile, providing insights into their potential as anticancer scaffolds. Twelve novel coumarin-based compounds were obtained in good yields (40-80%) and evaluated through in vitro, in silico, and in vivo assays. Among them, compound 7f displayed the highest antiproliferative potency and selectivity, with selectivity indices (SI) of 4.61 for B16F-10, 3.38 for HCT116, and 2.98 for 4T1, and an average SI of 2.18 across SCC-4, SCC-9, and SCC-25 cell lines. Mechanistic assays indicated that the cytotoxic effect of 7f is associated with oxidative stress-induced apoptosis, as evidenced by elevated ROS levels and activation of caspases 3/7. In vivo toxicity assessment in C57BL/6 mice revealed no significant changes in body weight, food intake, or macroscopic organ alterations at doses of up to 400 mg/kg, indicating a favorable safety profile. Additionally, in vitro biochemical evaluation and molecular docking suggested that 7f interacts with Pyruvate Kinase M2 (PKM2) and inhibits its glycolytic activity in a dose-dependent manner. Overall, the findings identify compound 7f as a selective and low-toxicity coumarin hybrid that exerts cytotoxic effects through ROS-mediated apoptosis, representing a promising lead structure for future optimization in anticancer drug development.