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Down-Regulation of lncRNA CKMT2-AS1 Predicts Poor Prognosis and Promotes Breast Cancer Progression
Dengfeng Xue1, Ruihong Hou2, Huijuan Xu1
1Department of Breast Surgery, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, People's Republic of China.
Breast Cancer (Dove Medical Press)
|January 1, 2026
Summary
Long non-coding RNA CKMT2-AS1 shows reduced expression in breast cancer, correlating with advanced disease. Upregulating CKMT2-AS1 suppresses tumor growth and metastasis, suggesting its potential as a prognostic biomarker.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) play crucial roles in various cancers.
- The specific function of lncRNA CKMT2-AS1 in breast cancer remains largely unexplored.
Purpose of the Study:
- To investigate the clinical significance of lncRNA CKMT2-AS1 in breast cancer.
- To elucidate the molecular mechanisms underlying CKMT2-AS1's role in breast cancer progression.
Main Methods:
- Assessed CKMT2-AS1 expression in breast cancer tissues and cell lines.
- Utilized Cox regression analysis for prognostic evaluation.
- Performed functional assays (proliferation, migration, invasion, apoptosis) and mechanistic studies (dual-luciferase reporter assays, bioinformatics, Western blot).
Main Results:
- CKMT2-AS1 expression was significantly decreased in breast cancer, correlating with advanced tumor stage and lymph node metastasis.
- Overexpression of CKMT2-AS1 inhibited breast cancer cell proliferation, migration, and invasion, while promoting apoptosis.
- CKMT2-AS1 directly interacts with miR-106b-5p, modulating MECP2 expression.
Conclusions:
- CKMT2-AS1 functions as a tumor suppressor in breast cancer by sequestering miR-106b-5p and affecting MECP2 levels.
- Reduced CKMT2-AS1 expression is associated with poor prognosis, identifying it as a potential prognostic biomarker for breast cancer.
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