Related Experiment Video
Updated: Jan 7, 2026
Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
Published on: December 17, 2015
Protocol for analyzing the microstructure of nuclear fiber layers by using structured illumination microscopy coupled
Miao Guan1, Kewei Li2, Xianglong Liu1
1The Interdisciplinary Research Group on Microscopic Imaging and Drug Development, Shenzhen Bay Laboratory, Shenzhen, China.
None:
Here, we present a protocol for quantitatively assessing the microstructure of nuclear fiber layers by immunolabeling of a nuclear skeleton protein lamin A through structured illumination microscopy (SIM) coupled with an innovative algorithm in Arivis 4D. We describe steps of sample preprocessing for SIM and imaging of the inner-nucleus skeleton network of lamin A. We then detail procedures for creating a novel algorithm in Arivis 4D software to quantitatively assess the microparameters of the nuclear skeleton in a high-resolution image.
