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miR156j-3p negatively regulates rice salt tolerance via ROS scavenging and ABA signaling

Shengxue Shao1, Guanjie Zhang1, Yao Wan1

  • 1Guangxi Key Laboratory of Agro-environment and Agro-products Safety, College of Agriculture, Guangxi University, Nanning, 530004, Guangxi, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, Nanning, 530004, Guangxi, China; Key Laboratory of Crop Cultivation and Physiology, Education Department of Guangxi Zhuang Autonomous Region National Demonstration, College of Agriculture, Guangxi University, Nanning, 530004, Guangxi, China.

Plant Physiology and Biochemistry : PPB
|January 1, 2026
PubMed

Insights

MicroRNA 156j-3p negatively impacts rice salt tolerance by targeting OsRePRP1.2. Downregulating this microRNA (miRNA) enhances salt tolerance through improved antioxidant activity and ABA signaling.

Area of Science:

  • Plant molecular biology
  • Abiotic stress responses
  • Gene regulation

Background:

  • MicroRNAs (miRNAs) are key regulators of plant responses to environmental stresses.
  • The miR156 family, particularly miR156-5p, is well-studied, but miR156-3p functions are less understood.
  • Understanding specific miRNA roles is crucial for improving crop resilience.

Purpose of the Study:

  • To investigate the role of miR156j-3p in rice (Oryza sativa) salt tolerance.
  • To identify the target gene of miR156j-3p involved in salt stress response.
  • To elucidate the molecular mechanisms underlying miR156j-3p-mediated salt tolerance.

Main Methods:

  • Genetic analysis using miR156j-3p downregulation (STTM156j-3p) and OsRePRP1.2 overexpression (RePRP1.2-ox) rice lines.
  • 5' RNA ligase-mediated rapid amplification of cDNA ends (5'-RLM-RACEs) assay to confirm direct target interaction.
  • Physiological assays measuring reactive oxygen species (ROS), malondialdehyde (MDA), and antioxidant enzyme activities.
  • Gene expression analysis of ABA signaling and ROS-scavenging pathways.

Main Results:

  • miR156j-3p negatively regulates rice salt tolerance by targeting OsRePRP1.2.
  • STTM156j-3p and RePRP1.2-ox plants showed enhanced salt tolerance.
  • Enhanced tolerance was linked to reduced H2O2 and MDA levels and increased antioxidant enzyme activity.
  • Genes in ABA signaling and ROS-scavenging pathways were upregulated in modified lines.

Conclusions:

  • miR156j-3p acts as a negative regulator of rice salt tolerance by targeting OsRePRP1.2.
  • The miR156j-3p/OsRePRP1.2 pathway influences salt tolerance via ROS scavenging and ABA signaling.
  • Modulating miR156j-3p offers a potential strategy for enhancing rice salt tolerance.
Keywords:
ABAOsRePRP1.2ROSRiceSalt tolerancemiR156j-3p

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