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Mycoplasma bovis immunogenic lipoprotein MbovP739 function as an inflammatory stimulating factor
Jiating Ma1, Shujuan Wang2, Doukun Lu2
1Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Ningxia University, Yinchuan, 750021, China; School of Life Sciences, Ningxia University, Yinchuan, 750021, China.
Abstract:
Mycoplasma bovis is a successful pathogen that causes bovine respiratory diseases worldwide. Due to its lack of cell wall, lipoproteins, key components of the mycoplasma membrane, are believed to play an important role in nutrient acquisition, induction of immune response, and adherence. This study evaluated the immunogenicity of nine lipoproteins and tested the ability of immunogenic lipoproteins to induce pro-inflammatory cytokine expression. The lipoproteins MbovP468, MbovP537, and MbovP739 of M. bovis were found to have higher immunogenicity and induced a greater amount of pro-inflammatory cytokines in RAW264.7 cells. RNA-sequencing demonstrated MbovP739 induced an immune response and significantly upregulated chemokines and cytokines expression in BoMac cells. The IL-17 signaling pathway and TNF signaling pathway were activated by MbovP739 in BoMac cells. We conclude that the immunogenic lipoprotein MbovP739 functions as an inflammatory stimulating factor.
Insights
Mycoplasma bovis lipoproteins are key to its survival. This study identified MbovP739 as a potent stimulator of pro-inflammatory cytokines, highlighting its role in bovine respiratory disease pathogenesis.
Area of Science:
- Veterinary Immunology
- Bacterial Pathogenesis
- Molecular Biology
Background:
- Mycoplasma bovis causes significant bovine respiratory disease globally.
- Lipoproteins are crucial membrane components in Mycoplasma bovis, involved in nutrient acquisition, immune response induction, and adherence.
- Understanding lipoprotein function is vital for controlling M. bovis infections.
Purpose of the Study:
- To evaluate the immunogenicity of nine Mycoplasma bovis lipoproteins.
- To determine the capacity of immunogenic lipoproteins to induce pro-inflammatory cytokine expression.
- To elucidate the molecular mechanisms underlying the immune response to specific lipoproteins.
Main Methods:
- Screening of nine Mycoplasma bovis lipoproteins for immunogenicity.
- Assessment of pro-inflammatory cytokine induction in RAW264.7 macrophage cells.
- RNA-sequencing analysis of bovine macrophages (BoMac cells) stimulated with identified lipoproteins.
- Analysis of activated signaling pathways (IL-17 and TNF) in BoMac cells.
Main Results:
- Three lipoproteins (MbovP468, MbovP537, MbovP739) exhibited higher immunogenicity.
- These lipoproteins induced significantly greater pro-inflammatory cytokine production in RAW264.7 cells.
- MbovP739 strongly induced an immune response in BoMac cells, upregulating chemokines and cytokines.
- The IL-17 and TNF signaling pathways were notably activated by MbovP739 in BoMac cells.
Conclusions:
- The Mycoplasma bovis lipoprotein MbovP739 demonstrates significant immunogenicity.
- MbovP739 acts as a potent inflammatory stimulating factor, driving pro-inflammatory cytokine expression.
- These findings identify MbovP739 as a potential target for therapeutic or diagnostic strategies against Mycoplasma bovis infections.
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