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Updated: Jul 8, 2026

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
Published on: August 18, 2018
Quantitative In Vitro and In Vivo Assays to Assess the Efferocytic Efficiency of Macrophages
Eduardo Gutseit1, Vinícius Amorim Beltrami1, Débora Gonzaga Martins1
1Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte-MG, Brazil.
Abstract:
Efferocytosis is a process in which specialized phagocytes, mainly macrophages, are responsible for recognizing and removing apoptotic cells from the inflammatory site, leading to tissue resolution, and preventing chronic inflammation. Herein we describe in detail a robust efferocytosis assay based on morphological criteria and flow cytometric for the quantification of efferocytic events and characterization of the engulfing efficiency of apoptotic cells by macrophage in vitro and in vivo, which can be used in many applications, including the development of genetic and pharmacological therapeutic targeting inflammation resolution.
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