Related Experiment Video
Updated: Jan 7, 2026
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Published on: January 12, 2021
A Tailing Method of Quality Control on MALBAC-Based Whole-Genome Amplification Products
Changyue Chen1, Jing Li2, ZengHui Xu3
1Department of Medical Research, Labway Company, Shanghai, China. changyue.chen@labway.cn.
Abstract:
Single-cell whole-genome sequencing (scWGS) has illuminated new perspectives on genetic diversity across different cellular conditions. Despite this, the amplification of DNA from sparsely populated formalin-fixed tissues during the whole-genome amplification (WGA) process remains a formidable challenge. We introduce an enhanced quality control protocol for WGA products generated using the multiple annealing and looping-based amplification cycles (MALBAC) technique. This approach presents a cost-efficient, high-throughput quality control method, delivering a precise assessment of WGA efficacy with 100% accuracy on a test set of 128 samples.
More Related Videos
10:12Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
Published on: May 15, 2018
13:24Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
Published on: April 11, 2016