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Updated: Jan 7, 2026
Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
Published on: July 29, 2016
Whole-Genome Amplification of Single Circulating Tumor Cells from Mice Xenografts
Nuria Estévez-Gómez1,2, Cristóbal Fernández-Santiago3, Roberto Piñeiro3
1CINBIO, Universidade de Vigo, Vigo, Spain. nuestevez@uvigo.es.
Abstract:
The genomic analysis of circulating tumor cells (CTCs) can help us identify their specific characteristics and reveal intratumor heterogeneity while also reducing the need for invasive sampling. However, studying single CTCs can be challenging, as whole-genome amplification (WGA) is needed to obtain enough genetic material for high-throughput sequencing. Here, we present a protocol for isolating single CTCs from mouse xenografts, followed by WGA using the Ampli1WGA Plus kit.
