Related Experiment Video
Updated: Jan 7, 2026

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
Published on: January 8, 2008
A Beginner's Guide to Immunohistochemistry Technique for Paraffin Sections
1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, Queens, NY, USA.
Abstract:
Immunohistochemistry is a robust technique in the field of pathology as well as basic science research in detecting an antigen of interest in any tissue. Here, we describe a detailed immunohistochemistry (IHC) technique utilizing liver tissue harvested from C57BL/6 mice. Mice were fed a high-fat diet for 22 weeks and developed fatty liver disease. The whole IHC process consists of tissue processing, embedding, sectioning, and staining. This chapter describes a detailed step-by-step IHC procedure to detect lipocalin-type prostaglandin D2 synthase (L-PGDS) protein in mice liver tissue.

