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Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
Published on: January 8, 2008
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A Beginner's Guide to Immunohistochemistry Technique for Paraffin Sections
1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, Queens, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 1, 2026
Summary
This study details a robust immunohistochemistry (IHC) method for detecting lipocalin-type prostaglandin D2 synthase (L-PGDS) protein in mouse liver tissue, particularly in models of fatty liver disease.
Area of Science:
- Pathology
- Immunology
- Biochemistry
Background:
- Immunohistochemistry (IHC) is a vital technique for antigen detection in biological tissues.
- Fatty liver disease is a condition that can be studied using animal models.
- Lipocalin-type prostaglandin D2 synthase (L-PGDS) plays a role in various physiological processes.
Purpose of the Study:
- To describe a detailed step-by-step immunohistochemistry (IHC) protocol.
- To optimize IHC for detecting L-PGDS protein in mouse liver tissue.
- To provide a method for analyzing liver tissue from mice with diet-induced fatty liver disease.
Main Methods:
- Harvesting liver tissue from C57BL/6 mice fed a high-fat diet for 22 weeks.
- Tissue processing, including fixation, embedding, and sectioning.
- Performing immunohistochemical staining to detect L-PGDS protein.
Main Results:
- Successful application of the IHC technique to detect L-PGDS protein in mouse liver.
- Demonstration of the method's utility in a fatty liver disease model.
- Detailed protocol steps for reproducible results.
Conclusions:
- The described IHC method is effective for L-PGDS detection in mouse liver.
- This protocol facilitates the study of L-PGDS in the context of fatty liver disease.
- The technique is valuable for both basic science research and pathology.
