Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence
Herui Wang1, Ryan L Pangilinan1, Yan Zhu2
1Department of Biological Sciences, St. John's University, Queens, NY, USA.
None:
Tyramide signal amplification (TSA) is an enzyme-mediated method to enhance the immunohistochemical detection of protein, nucleic acid, or other molecules in situ. Here we describe immunofluorescence detection of a low-abundance cytokine receptor, interleukin-17 receptor B (IL17RB) in U2OS cells, using tyramide signal amplification. In addition, we present a tyramide signal amplification compatible double-color immunostaining protocol using primary antibodies from the same host species. Those applications allow detection of cellular proteins with enhanced sensitivity and add flexibility to primary antibody selection in double or multicolor immunofluorescence staining assays.
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