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Detection of Ferroptosis by Liperfluo and BODIPY™ 581/591 C11

Cristina Aguilar Flores1, Wan Seok Yang2

  • 1Department of Biological Sciences, St. John's University, Queens, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 1, 2026
PubMed

Keywords:
BODIPY-C11Erastin, RSL3FerroptosisLiperfluoLipid peroxides

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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...