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Differentiation and Polarization of THP-1 Cells into M1 and M2 Macrophages for Cancer Research
Abeir Ahmed1, Patricia Rozario1, Yan Zhu2
1Department of Biological Sciences, St. John's University, Queens, NY, USA.
Abstract:
The human monocytic leukemia THP-1 cell line has been frequently used for cellular inflammation studies and cancer research since it can be differentiated into macrophages and polarized into different functional subtypes. THP-1-derived macrophages are considered viable substitutes for primary monocyte-derived macrophages in many in vitro studies, including cancer research. Various experimental conditions have been reported for THP-1 differentiation and polarization. Here, we present a reproducible method of THP-1 differentiation and polarization into M1 and M2 macrophages based on commonly used stimulants and markers of differentiation.
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