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Updated: Jan 7, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Published on: October 15, 2021
Method for Measuring the Kinetic Stability of Peptides Bound onto MHC Class I Using MALDI-TOF Mass Spectrometry
George Mavridis1, Manousos Makridakis2, Jerome Zoidakis3
1Department of Chemistry, National and Kapodistrian University of Athens, Athens, Greece.
Abstract:
The kinetic stability of peptides bound onto MHC class I molecules is a critical parameter that helps shape their interaction with immune receptors and consequently their antigenic potential. We present here a method for measuring the kinetic stability and sensitivity to proteolytic degradation of peptides bound onto MHC class I molecules after in vitro refolding, by analyzing the time-resolved MALDI-TOF Mass Spec signal from the peptide, using the whole MHC-I/peptide complex in situ. This approach can provide important information on the dynamic nature of the MHC-peptide interaction, the kinetic half-life of binding and the sensitivity of the peptide to external proteolytic digestion or other modifications.
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