Cellular Assays to Monitor Antigen Cross-presentation In Vitro.
François-Xavier Mauvais1,2, Peter van Endert3,4
1Université Paris Cité, Paris, France. francois-xavier.mauvais@inserm.fr.
Methods in Molecular Biology (Clifton, N.J.)
|January 1, 2026
Summary
This study details in vitro antigen cross-presentation assays using MutuDC and HEK 293T cell lines. These methods help evaluate antigen cross-presentation efficiency and mechanisms for immune research.
Area of Science:
- Immunology
- Cell Biology
Background:
- Antigen cross-presentation is vital for adaptive immunity against tumors and pathogens.
- It involves presenting extracellular antigens via MHC-I to CD8+ T cells.
- Assays are crucial for understanding cross-presentation efficiency and mechanisms.
Purpose of the Study:
- To describe protocols for in vitro antigen cross-presentation assays.
- To utilize MutuDC (murine dendritic cell line) and HEK 293T cells for these assays.
- To provide methods for evaluating antigen cross-presentation in different cell types.
Main Methods:
- Utilized MutuDC, a murine immortalized type 1 conventional dendritic cell line.
- Employed human embryonic kidney HEK 293T cells engineered for immune complex cross-presentation via Fc receptor.
- Developed in vitro antigen cross-presentation assay protocols.
Main Results:
- Established protocols for using MutuDC and HEK 293T cells in antigen cross-presentation assays.
- Demonstrated the utility of these cell lines for studying cross-presentation mechanisms.
- Provided a framework for evaluating antigen cross-presentation efficiency.
Conclusions:
- MutuDC and engineered HEK 293T cells are valuable tools for in vitro antigen cross-presentation studies.
- These assays facilitate the investigation of molecular and cellular mechanisms of cross-presentation.
- The described methods aid in assessing the efficiency of antigen cross-presentation.
