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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Cell Painting Protocol to Characterize Morphological Profiles of Large Compound Collections Using the EU-OPENSCREEN

Christopher Wolff1, Martin Neuenschwander2, Michelle Müller2

  • 1Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP), Campus Berlin-Buch, Berlin, Germany. wolff@fmp-berlin.de.

Methods in Molecular Biology (Clifton, N.J.)
|January 1, 2026
PubMed
Summary
This summary is machine-generated.

Cell Painting assay offers unbiased characterization of chemical compounds by analyzing detailed cellular morphology. This validated protocol links compound mechanisms to observed phenotypic profiles for robust screening.

Keywords:
Cell PaintingEU-OPENSCREEN libraryFluorescent dyesMorphological profiling

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Area of Science:

  • Cellular imaging and phenotypic screening
  • Chemical biology and drug discovery

Background:

  • Morphological profiling using Cell Painting assay revolutionizes phenotypic screening.
  • Enables unbiased characterization of cellular perturbations caused by small chemical compounds.

Purpose of the Study:

  • Establish and validate a Cell Painting protocol for screening EU-OPENSCREEN compound libraries.
  • To establish robust and reproducible links between compound mechanisms and phenotypic profiles.

Main Methods:

  • Detailed description of the Cell Painting procedure.
  • Image analysis techniques for morphological profiling.
  • Downstream data processing for phenotypic data.

Main Results:

  • Validated Cell Painting protocol for large-scale compound library screening.
  • Demonstrated ability to link compound target/pathway to phenotypic profiles.
  • Established a framework for reproducible phenotypic screening.

Conclusions:

  • Cell Painting is a powerful tool for phenotypic screening and chemical biology.
  • The validated protocol enables efficient screening of compound libraries.
  • Facilitates understanding of compound mechanisms through morphological profiling.