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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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The Transcriptional Role of Circular RNAs in Drug Resistance by Modulating the miRNA/mRNA Axis

Mohammad H Ghazimoradi1, Amirmohammad Moghadam1, Parastoo Akbarabadi1

  • 1Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Experientia Supplementum (2012)
|January 1, 2026
PubMed

Insights

Circular RNAs (CRNA) regulate microRNAs (miRNA) to control messenger RNA (mRNA) degradation, impacting cancer drug resistance. This CRNA/miRNA/mRNA axis offers new insights into overcoming treatment challenges.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cancer Research

Background:

  • Drug resistance significantly impairs cancer treatment efficacy and patient survival.
  • The precise mechanisms driving cancer drug resistance initiation and progression remain incompletely understood.
  • Noncoding RNAs, particularly circular RNAs, are increasingly recognized as critical epigenetic regulators implicated in various diseases, including cancer.

Purpose of the Study:

  • To elucidate the role of circular RNA (CRNA) in regulating microRNA (miRNA) activity within the context of cancer drug resistance.
  • To investigate the CRNA/miRNA/mRNA regulatory axis and its contribution to the development of cancer drug resistance.
  • To explore potential therapeutic targets by understanding the molecular mechanisms of CRNA-mediated drug resistance.

Main Methods:

  • Bioinformatic analysis to identify deregulated CRNA, miRNA, and mRNA expression profiles in resistant cancer models.
  • Experimental validation of CRNA-miRNA interactions and their impact on mRNA targets.
  • Functional assays to assess the role of the CRNA/miRNA/mRNA axis in conferring resistance to common cancer therapeutics.

Main Results:

  • Identified a novel regulatory axis where specific circular RNAs (CRNAs) modulate microRNA (miRNA) function.
  • Demonstrated that this CRNA/miRNA/mRNA interaction influences messenger RNA (mRNA) degradation pathways critical for drug resistance.
  • Observed dysregulation of multiple oncogenic and tumor-suppressive pathways mediated by this axis in common cancer types.

Conclusions:

  • The CRNA/miRNA/mRNA axis represents a key epigenetic mechanism driving cancer drug resistance.
  • Targeting this axis holds potential for developing novel strategies to overcome therapeutic resistance in cancer patients.
  • Further research into CRNA-mediated epigenetic regulation is crucial for advancing cancer treatment outcomes.
Keywords:
Circular RNADrug resistanceNoncoding RNAmRNAmiRNA

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