Isolation and characterization of CD73+CD39+CD146+ mesenchymal stem cell subset from bone marrow.

Area of Science:

• Cell biology
• Hematology
• Regenerative medicine

Background:

• Mesenchymal stem cells (MSCs) are crucial for regulating hematopoiesis.
• A specific MSC subset expressing CD73, CD39, and CD146 has enhanced osteo-chondrogenic potential and adenosine production.
• CD146 is associated with pericytes, suggesting a perivascular localization for this MSC subset.

Purpose of the Study:

• To identify and characterize the CD73+CD39+CD146+ MSC subset in bone marrow (BM).
• To investigate the presence of this MSC subset in mobilized peripheral blood.

Main Methods:

• Bone marrow (BM) cells were expanded and analyzed for CD73, CD39, and CD146 expression via flow cytometry.
• Cell sorting was performed using BDFACS AriaTM Fusion.
• The purified MSC subset was assessed for stem cell characteristics and perivascular localization in BM sections. Peripheral blood MSCs were obtained via apheresis.

Main Results:

• A distinct MSC subset with a CD45-CD73+CD39+CD146+ phenotype was identified and purified from culture-expanded BM up to passage 4.
• This subset demonstrated self-renewal and multipotency capabilities.
• The CD73+CD39+CD146+ MSCs were found in perivascular areas of BM and also present in mobilized leukopaks.

Conclusions:

• The CD73+CD39+CD146+ MSC subset possesses self-renewal and multipotency properties.
• This specific MSC subset is localized in perivascular regions of the bone marrow.
• The presence of this subset in mobilized leukopaks suggests potential for cell therapy applications.