C6‑HSL enhances BPA tolerance in Pseudomonas asiatica: an integrative Raman‑DIP and transcriptomic study

Area of Science:

• Environmental Microbiology
• Bacterial Stress Response
• Bioremediation

Background:

• Bisphenol A (BPA) is a widespread environmental contaminant with significant biotoxicity to microorganisms.
• Understanding mechanisms to enhance bacterial stress resistance is crucial for environmental detoxification.
• BPA exposure negatively impacts bacterial cell viability and gene expression.

Purpose of the Study:

• To investigate the role of C6-HSL in enhancing bacterial resistance to Bisphenol A (BPA) toxicity.
• To elucidate the molecular mechanisms underlying C6-HSL-mediated protection against BPA stress.
• To explore the combined application of Raman-DIP, transcriptomics, and enzyme activity assays for mechanism analysis.

Main Methods:

• Transcriptomic analysis to identify gene expression changes under BPA stress and C6-HSL treatment.
• Measurement of cell viability and intracellular reactive oxygen species (ROS) levels.
• Assay of ATP content and related enzyme activities.

Main Results:

• BPA stress downregulates over 59% of functional genes, reducing cell viability and ATP content.
• 10 μM C6-HSL significantly enhances BPA resistance, increasing cell viability by 1.13-fold.
• C6-HSL upregulates genes involved in antioxidant defense, amino acid synthesis, and energy supply, reducing ROS by 17.28% and increasing ATP content by 12.21%.

Conclusions:

• C6-HSL effectively enhances bacterial resistance to Bisphenol A toxicity by modulating antioxidant, amino acid, and energy systems.
• This study provides the first evidence of C6-HSL's function in improving bacterial BPA resistance.
• The findings offer methodological references and theoretical support for enhancing microbial detoxification capabilities.