The Effect of 6-Gingerol on Human AML Cell Lines

Area of Science:

• Hematology
• Oncology
• Pharmacology
• Traditional Chinese Medicine

Background:

• Acute myeloid leukemia (AML) is a severe blood cancer with limited treatment options.
• 6-Gingerol, a bioactive compound, has demonstrated anti-cancer properties in various studies.
• The therapeutic potential of 6-Gingerol in AML has not been extensively investigated.

Purpose of the Study:

• To investigate the anti-leukemic effects of 6-Gingerol on acute myeloid leukemia (AML) cells.
• To explore the underlying molecular mechanisms of 6-Gingerol's action in AML.
• To identify potential therapeutic targets for 6-Gingerol in AML treatment.

Main Methods:

• Cell proliferation, clonogenic capacity, cell cycle, and apoptosis were assessed using CCK-8, colony formation assays, flow cytometry, and Transwell migration assays.
• Intracellular reactive oxygen species (ROS) levels and protein expression (Bcl-2, caspase3, MAPK, p-MAPK) were analyzed via fluorescent probe staining and Western blot.
• Bioinformatics databases and enrichment analysis (GO, KEGG) were employed to identify 6-Gingerol targets in AML.

Main Results:

• 6-Gingerol significantly inhibited AML cell proliferation, colony formation, and invasive potential.
• Treatment with 6-Gingerol induced G1 cell-cycle arrest and apoptosis, increased ROS levels, and elevated caspase 3, MAPK, and p-MAPK expression.
• Bioinformatic analysis identified 67 overlapping targets, highlighting MAPK signaling and ROS pathways, with NFKB1 as a key hub gene.

Conclusions:

• 6-Gingerol exhibits potent anti-leukemic activity against AML cells in vitro.
• The findings suggest that 6-Gingerol modulates cell cycle, apoptosis, and ROS pathways in AML.
• 6-Gingerol shows promise as a novel therapeutic agent derived from Traditional Chinese Medicine for AML treatment.