Related Experiment Video
Updated: Jan 7, 2026
Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula
Published on: January 7, 2019
Microbial transformation of cycloastragenol by Penicillium spinulosum AS3.149
Xin-Yu Cai1, Ji-Mei Liu1, Jun-Gui Dai1
1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, NHC Key Laboratory of Biosynthesis of Natural Products, CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs, and Beijing Key Laboratory of Non-Clinical Drug Metabolism and PK/PD Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Abstract:
Two new metabolites, 29-demethyl-3-ene-cycloastragenol (2) and 1α,12α-dihydroxy-cycloastragenone (3), along with six known compounds (4-9) were obtained from the microbial transformation of cycloastragenol (1) by Penicillium spinulosum AS3.149. The structures of these metabolites were determined by extensive spectroscopic (IR, UV, HRESIMS, 1D and 2D NMR) data analyses. Compound 2 features a 29-nor-triterpenic skeleton, possibly formed through sequential oxidation, decarboxylation, and dehydration reactions. Biological assays revealed that compound 6 exhibited significant cytotoxic activity against the K562 cell line with an IC50 value of 1.0 μM.
More Related Videos
08:00Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens
Published on: October 27, 2023
11:47Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna
Published on: February 1, 2022
Related Concept Videos
01:26Transformation
02:50Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...